Upon entering the Cell Types Tool, you will be presented with an overview of the tool and its role in working to establish "definitive phenotypes" for a variety of cell types commonly assayed in immunophenotyping assays. Beneath that description are links to several different groupings of cells or development pathways: B Cell Maturation, General Immune Subsets, Hematopoiesis, T Cell Maturation and T Cell Subsets.
Clicking the title of, or view button near, any of these subsets/groups will open the page specific to that subset/group. In this case, we'll use the "General Immune Subsets" group as an example:
At the top of the "General Immune Subsets" page we are first presented with a literature review that establishes FluoroFinder's definitive phenotype for that cell population. Clicking "Read more" beneath the initially visible block of text will expand the entire literature review, providing detailed descriptions and phenotypes for the "General Immune Subset" grouping
Beneath the literature review is a graphical representation (Interactive Image) of each of the described cell types. The developmental path of the various subsets are depicted, including defining markers.
Clicking on any of the cell type headers (e.g. Non-Classical Monocytes) will open a pop-up window containing details about that population, including a description of the cell type, where they are located in the body, and a variety of markers that can be used in immunophenotyping and subsetting the population.
Each individual marker (e.g. CD34-) can be clicked to navigate to a page with more details about that marker and links to associated products.
Beneath the Interactive Image, FluoroFinder also may provide a representative gating structure for phenotypic identification of your population after data acquisition via your platform of choice. This gating structure can be used to guide target population identification in your final experiment.
NOTE: The function of the cell is the ultimate indicator of ontology, and a consensus is still required on the most accurate downstream functional assays for functional validation that might yet add to the completeness of this tool. To submit a peer-reviewed publication that would contribute to updating this tool, please contact us.